Because the 16S profiles through the gut seem quite diverse for intracellular and extracellular DNA [33], qPCR is anticipated to reflect the NGS-qualified Neighborhood structure both equally quantitatively and qualitatively much more carefully than flow cytometry.
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Importantly, qPCR-centered quantitative microbiome profiling enjoys the next conceptual and realistic benefits above other ways:
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Using relative abundance from subsequent technology sequencing (NGS) read more information can result in misinterpretations of microbial Group constructions as because of compositionality, the relative abundances in the taxa staying mutually dependent. Consequently an increase of 1 taxon inevitably results in the concurrent lower of another(s). Because the alterations of factors are mutually dependent, high false discovery prices come about when compositional information are analyzed working with conventional statistical methods [one].
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